Product Outline:

• A µ-Slide with a porous glass membrane for transmigration and transport studies under both static and flow conditions
• Brilliant optical quality through the thin, porous glass membrane
• Full access to the apical and basal sides of adherent cells in numerous applications
• Different pore sizes available for various cell types
• Suitable for the establishment of lung models with air-liquid interface (ALI)

Applications

• Trans-endothelial migration under flow conditions
• Co-cultivation of cell layers and transport in 2D or in a 3D gel matrix
• Apical-basal cell polarity assays
• Skin and lung models with liquid-air interface
• Cell barrier model assays with apical-basal gradients
• Cell migration assays based on filters and porous membranes

A Perfusable Channel µ-Slide With 3 x 7 Wells for Long-Term Spheroid Cultivation and High-End Microscopy

Product outline:

• Ideal for the long-term cultivation and perfusion of 3D spheroids or organoids on the Bioinert surface
• Flexible preparation for perfusion: spheroids can be transferred or created directly in the wells
• Imaging chamber with excellent optical quality for high-resolution microscopy

Applications

• Cultivation and high-resolution microscopy of three-dimensional cell aggregates (e.g., spheroids and organoids)
• For use with the ibidi Pump System or any other pump device with Luer connectors
• Perfusion of spheroids for fresh medium supply during long-term cultivation and access to spheroid growth kinetics
• Spheroid generation directly in the wells
• Spheroid retrieval for downstream processing (e.g., immunofluorescence, histology, and biochemical assays)
• Perfusion of cell monolayers (adherent cells), suspension cells, or co-cultures
• Organ-on-a-chip setups with up- and downstream metabolization
• Live cell imaging and microscopy of 3D cell aggregates
• Immunofluorescence staining and high-resolution fluorescence microscopy of living and fixed cells and cell aggregates

Nanoscale Imaging Has Never Been Easier

Collect nanoscale topography data on your benchtop in three clicks with the nGauge AFM.

ICSPI has integrated all of the components of a traditional AFM onto a single 1 mm x 1 mm chip to create the world’s first single-chip AFM. That means that 250 AFMs can fit on the face of a penny.

The nGauge AFM uses micro-electro-mechanical systems (MEMS) to control the X, Y and Z position of the tip. This means that no alignment of lasers is required.

The nGauge is the smallest AFM in the world. Because the entire unit weighs less than 500 grams, the nGauge rejects building vibrations, so no vibration isolation table is needed, which makes the nGauge a true benchtop AFM.

 

What can I use AFM for?

AFM provides topographical data of a surface. That means that you can look at the shape and size of individual features, such as the pits on a DVD, or look at the particle density, such as the number of nanoparticles in an area.

The nGauge AFM can be used to investigate surfaces where the features are up to 10 µm tall. It’s tricky to pinpoint a lower limit, but the RMS noise in the vertical (z) direction of the nGauge is 1 nanometre (nm). So, depending on your requirements, features as small as 5–10 nm can be imaged with the nGauge with acceptable accuracy.

To put what a nanometer is into perspective, a quarter (25-cent coin) is about 2 cm wide. An E. coli bacterium cell is 2 µm wide (10,000× smaller than a quarter). And the diameter of a DNA helix is 2 nm (1,000× smaller than a bacterium).