Product outline:

• A µ-Slide used to investigate angiogenesis in tube formation assays. Also ideal for 3D cell culture and immunofluorescence staining.
• Complete solution for tube formation experiments, requiring only a few steps from sample preparation to image analysis
• Brilliant visualization without meniscus formation, and with all cells in one focal plane
• Can be used with a broad range of gels (e.g., Matrigel®, collagen, and agarose)
• Cost-effective experiments, requiring only 10 µl of gel per well

Applications

• Tube formation assays
• 3D cell culture
• Immunofluorescence staining
• Sprouting assays
• Live cell imaging of attached cells

Product outline:

• Investigate chemotaxis of fast or slow migrating adherent cells and non-adherent cells in 2D or 3D gel matrices
• Real-time chemotaxis measurement in real-time in a 2D or 3D environment
• Stable chemotactic gradients for long-term experiments
• Reproducible results with reliable and user-independent data

Applications

• 2D and 3D chemotaxis assays with fast or slow migrating cells
• Live cell imaging using inverted microscopy
• Chemotaxis of neutrophils, lymphocytes, and monocytes
• 3D chemotaxis of leukocytes or cancer cells in an ECM-like matrix
• Invasion assays of tumour cells in Matrigel™
• Chemotaxis measurements of adherent and non-adherent cells

Product outline:

• Channel slides with different heights, volumes, and coatings especially suited for flow applications
• Large area of uniform shear stress
• Easy connection using Luer adapters
• Homogeneous cell distribution with defined optical pathway

Applications

• Adherent cells under flow conditions
• Cell culture (static or stop-flow)
• Simulation of blood vessels with endothelial cells
• High-resolution microscopy of living and fixed cells

A µ-Slide With One Channel and Three Wells for Culturing Cells on a 3D Gel Matrix With Defined Flow

Product outline:

• Create a perfusable cell monolayer on a gel matrix for various flow and shear stress applications
• Establish and image endothelial barriers under in vivo-like conditions—no artificial filters or membranes
• Easy handling: samples are placed into the open wells; perfusion is applied after closing the wells

Applications

• Culture of adherent cells on a 3D gel matrix under flow conditions
• Transendothelial migration studies under flow conditions
• Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
• Simulation of blood vessels with endothelial cell culture on a soft substrate
• Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
• Apical-basal cell polarity assays
• Cell barrier model assays with apical-basal gradients
• Cell boundary assays without filters or porous membranes
• Rolling and adhesion assays using leukocytes
• Inflammation studies
• Transport studies