Ibidi

Product outline:

• Channel slides with different heights, volumes, and coatings especially suited for flow applications
• Large area of uniform shear stress
• Easy connection using Luer adapters
• Homogeneous cell distribution with defined optical pathway

Applications

• Adherent cells under flow conditions
• Cell culture (static or stop-flow)
• Simulation of blood vessels with endothelial cells
• High-resolution microscopy of living and fixed cells

A µ-Slide With One Channel and Three Wells for Culturing Cells on a 3D Gel Matrix With Defined Flow

Product outline:

• Create a perfusable cell monolayer on a gel matrix for various flow and shear stress applications
• Establish and image endothelial barriers under in vivo-like conditions—no artificial filters or membranes
• Easy handling: samples are placed into the open wells; perfusion is applied after closing the wells

Applications

• Culture of adherent cells on a 3D gel matrix under flow conditions
• Transendothelial migration studies under flow conditions
• Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
• Simulation of blood vessels with endothelial cell culture on a soft substrate
• Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
• Apical-basal cell polarity assays
• Cell barrier model assays with apical-basal gradients
• Cell boundary assays without filters or porous membranes
• Rolling and adhesion assays using leukocytes
• Inflammation studies
• Transport studies

Product Outline:

• A µ-Slide with a porous glass membrane for transmigration and transport studies under both static and flow conditions
• Brilliant optical quality through the thin, porous glass membrane
• Full access to the apical and basal sides of adherent cells in numerous applications
• Different pore sizes available for various cell types
• Suitable for the establishment of lung models with air-liquid interface (ALI)

Applications

• Trans-endothelial migration under flow conditions
• Co-cultivation of cell layers and transport in 2D or in a 3D gel matrix
• Apical-basal cell polarity assays
• Skin and lung models with liquid-air interface
• Cell barrier model assays with apical-basal gradients
• Cell migration assays based on filters and porous membranes

A Perfusable Channel µ-Slide With 3 x 7 Wells for Long-Term Spheroid Cultivation and High-End Microscopy

Product outline:

• Ideal for the long-term cultivation and perfusion of 3D spheroids or organoids on the Bioinert surface
• Flexible preparation for perfusion: spheroids can be transferred or created directly in the wells
• Imaging chamber with excellent optical quality for high-resolution microscopy

Applications

• Cultivation and high-resolution microscopy of three-dimensional cell aggregates (e.g., spheroids and organoids)
• For use with the ibidi Pump System or any other pump device with Luer connectors
• Perfusion of spheroids for fresh medium supply during long-term cultivation and access to spheroid growth kinetics
• Spheroid generation directly in the wells
• Spheroid retrieval for downstream processing (e.g., immunofluorescence, histology, and biochemical assays)
• Perfusion of cell monolayers (adherent cells), suspension cells, or co-cultures
• Organ-on-a-chip setups with up- and downstream metabolization
• Live cell imaging and microscopy of 3D cell aggregates
• Immunofluorescence staining and high-resolution fluorescence microscopy of living and fixed cells and cell aggregates